解剖学报

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 194-198.doi: 10.3969/j.issn.0529-1356.2013.02.009

• 细胞和分子生物学 • 上一篇    下一篇

纤维蛋白凝胶承载内皮祖细胞移植梗死心肌后细胞自噬变化

张丹  王海杰*  谭玉珍  王强利  伍金红  李志华  权哲    

  1. 复旦大学上海医学院人体解剖学与组织学胚胎学系，上海 200032
  • 收稿日期:2012-10-09 修回日期:2012-11-26 出版日期:2013-04-06 发布日期:2013-04-06
  • 通讯作者: 王海杰 E-mail:hjwang@shmu.edu.cn
  • 基金资助:

    教育部高等学校博士点基金（200802460044）；国家自然科学基金（30570948）；上海市科委国际合作基金（11540702500）

Autophagic changes of the endothelial progenitor cells carried with fibrin glue after transplantation into the infracted myocardium

ZHANG Dan WANG Hai-jie* TAN Yu-zhen WANG Qiang-li WU Jin-hong LI Zhi-hua Quan Zhe    

  1. Department of Anatomy, Histology and Embryology, Shanghai Medical School of Fudan University, Shanghai 200032, China
  • Received:2012-10-09 Revised:2012-11-26 Online:2013-04-06 Published:2013-04-06

摘要：

目的 观察心肌梗死后移植内皮祖细胞（EPCs）时的细胞自噬变化，探讨自噬维持移植细胞存活和纤维蛋白凝胶保护细胞的作用。方法 通过结扎左冠状动脉的前降支建立大鼠心肌梗死模型后，在正常区、梗死边缘区和梗死区分别注射从人脐带血中分选的EPCs。2 h后取注射处组织，作半薄切片，观察纤维蛋白凝胶承载的EPCs分布。定位后作超薄切片，透射电镜下观察EPCs的自噬结构变化以及纤维蛋白凝胶与EPCs和心肌的相容性。结果 与正常区相比，梗死边缘区发生自噬的EPCs增多，细胞内自噬结构也增多。梗死区的移植细胞的自噬显著增强，有的细胞坏死或凋亡。纤维蛋白凝胶与移植细胞和心肌的相容性良好，移植的EPCs在纤维蛋白凝胶中充分伸展，有的EPCs与心肌细胞黏附。结论 在梗死边缘区移植EPCs后，轻度缺血刺激细胞发生自噬，这有利于维持移植细胞存活。纤维蛋白凝胶承载EPCs可避免细胞丢失和促进存活。

关键词: 自噬 ,  内皮祖细胞 ,  纤维蛋白 , 心肌梗死 , 细胞移植 , 透射电镜 , 大鼠

Abstract:

Objective To investigate autophagic changes endothelial progenitor cells (EPCs) carried with fibrin glue after transplantation into the infracted myocardial and to explore effects of autophagy on maintaining the implanted cells to survive and fibrin on protecting the cells. Methods The model of myocardial infarction was established with ligating the anterior descending branch of the left coronary artery of rats. The EPCs sorted from human umbilical cord blood were injected into the myocardium at the normal region, periphery of the infarcted region and infarcted region. After transplantation for two hours, the tissues at injection sites were removed, the semithin sections were prepared. Distribution of the EPCs carried with fibrin glue were examined. After positioning the implanted cells, the ultrathin sections were prepared. The changes of the autophagic structures in EPCs and compatibility of fibrin with EPCs and myocardium were evaluated. Results Compared with the normal region, the autophagic EPCs in the periphery of the infarcted region increased, and the autophagic structures in the cells increased. In the infarcted region, EPC autophagy enhanced significantly, and necrosis or apoptosis occurred in some cells. Compatibility of fibrin with EPCs and myocardium was good. The implanted cells in fibrin glue extended well, some EPCs adhered to cardiaomyocytes. Conclusion When EPCs are transplanted into the periphery of the infarcted region, mild ischemia induces autophagy of the cells, which is beneficial for maintaining survival of the transplanted cells. Carrying EPCs with fibrin glue may avoid of cell lose and promote cell survival.

Key words:  Autophagy , Endothelial progenitor cell , Fibrin , Myocardial infarction , Cell transplantation , Transmisson electron microscope , Rat

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